RNA
Part:BBa_K3120001:Design
Designed by: Ruoyi Xue Group: iGEM19_BM-AMU (2019-08-09)
N-cadherin's 20nt target sequence+ sgRNA scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene target site which selected must be a DNA sequence of about 20 nt that satisfy two conditions: 1.This sequence is unique in the genome. 2.The gene target site is immediately upstream of the PAM (Protospacer Adjacent Motif) region.
Source
Designed according to the CDS sequence of the N-cadherin gene and submitted to The Beijing Genomics Institute for synthesis.